Analysis Note

ControlPOSITIVE CONTROL: brain (sympathetic nerve terminals) and adrenal glands.

Application

Immunohistochemistry: 1:2,000-1:4,000.

Optimal working dilutions must be determined by end user.

This Anti-Tyrosine Hydroxylase Antibody is validated for use in IH for the detection of Tyrosine Hydroxylase.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & ReceptorsNeuronal & Glial Markers

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide corresponding to amino acids 32-47 of rat Tyrosine Hydroxylase.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit serum. Lyophilized. Reconstitute with 50 µL of sterile distilled water. Centrifuge to remove any insoluble material. Contains no preservative.

Specificity

Tyrosine Hydroxylase. Immunohistochemical distribution throughout the brain shows that staining is restricted to those neurons known to contain the enzyme. The antibody has proven useful in staining catecholaminergic neurons. AB5986 stains these neurons intensely, including dendritic processes and fine nerve terminals.

Storage and Stability

Maintain lyophilized material frozen at -20°C to -70°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1, ACS or better grade) can be added for additional stability.